Hotspot

trnF-GAA-ndhJ

LSC · 47734-48274 · 541 bp

Back to Melissa

Pi 0.016

MarkerSeek score 67.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 13
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	682-792 bp	1.0	81.3
trnF-GAA-ndhJ_p2	GAGGGTGGGATGCTACATTG	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	711-821 bp	1.0	81.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	646-756 bp	1.0	81.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	683-793 bp	1.0	80.7
trnF-GAA-ndhJ_p5	GAGGGTGGGATGCTACATTG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	712-822 bp	1.0	80.5