Hotspot

psbM-trnD-GUC

LSC · 29352-29869 · 518 bp

Back to Melissa

Pi 0.00386

MarkerSeek score 53.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 3
pi: 0.00386
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	AGTTCAATCGGTTAGAGCACC	42.9	47.6	56.4	58.0	652 bp	1.0	51.3
psbM-trnD-GUC_p2	AGCGGTAGGAACTAGAATGA	AGTTCAATCGGTTAGAGCACC	45.0	47.6	55.4	58.0	644 bp	1.0	51.1
psbM-trnD-GUC_p3	AGTAGAAAAGCGGTAGGAACT	TGCCAGATCCCTTATTTCCC	42.9	50.0	56.4	56.9	681 bp	1.0	51.1
psbM-trnD-GUC_p4	AGCGGTAGGAACTAGAATGA	TGCCAGATCCCTTATTTCCC	45.0	50.0	55.4	56.9	673 bp	1.0	50.9
psbM-trnD-GUC_p5	AGTAGAAAAGCGGTAGGAACT	GCCAGATCCCTTATTTCCCTG	42.9	52.4	56.4	58.1	680 bp	1.0	50.4