Hotspot

atpI-rps2

LSC · 15196-15419 · 224 bp

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Pi 0.0134

MarkerSeek score 60.80

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 4
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GGAAACCCCCTATTTGCCAA	ATTTCGATTCCAGCGAACGA	50.0	45.0	58.1	58.0	432 bp	1.0	80.3
atpI-rps2_p2	GGAAACCCCCTATTTGCCAA	TTTCGATTCCAGCGAACGAT	50.0	45.0	58.1	58.0	431 bp	1.0	80.3
atpI-rps2_p3	TTGGAAACCCCCTATTTGCC	ATTTCGATTCCAGCGAACGA	50.0	45.0	58.1	58.0	434 bp	1.0	80.3
atpI-rps2_p4	TTGGAAACCCCCTATTTGCC	TTTCGATTCCAGCGAACGAT	50.0	45.0	58.1	58.0	433 bp	1.0	80.3
atpI-rps2_p5	CCATGTACTTGGAAACCCCC	TTTCGATTCCAGCGAACGAT	55.0	45.0	58.2	58.0	441 bp	1.0	79.5