Hotspot

ndhF-rpl32

SSC · 117987-119319 · 1333 bp

Back to Meliosma

Pi 0.316

MarkerSeek score 76.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1333
variable_sites: 282
pi: 0.316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAATTGGTCGTGTGAAC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	2555 bp	0.5	59.6
ndhF-rpl32_p2	CTGCAATTGGTCGTGTGAAC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	2556 bp	0.5	59.6
ndhF-rpl32_p3	CTGCAATTGGTCGTGTGAAC	TTTACCGCTGCCCAATATCC	50.0	50.0	58.0	58.0	1936 bp	0.5	59.5
ndhF-rpl32_p4	CTGAATAGACAGATCGGCCG	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.9	58.0	2224 bp	0.5	59.5
ndhF-rpl32_p5	CTGAATAGACAGATCGGCCG	CAGCGTGTCTACCGATTTCA	55.0	50.0	57.9	58.0	2225 bp	0.5	59.5