Hotspot

rpl20-rps12

LSC · 74107-74886 · 780 bp

Back to Meliosma

Pi 0.16

MarkerSeek score 83.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 120
pi: 0.16
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ATCCGAGTGATCCACAAACG	TGTTGATCTTGTAGCGGTCG	50.0	50.0	58.0	58.0	1300 bp	0.5	59.8
rpl20-rps12_p2	TCCGGGGAACTCTGTTTAGT	TGTTGATCTTGTAGCGGTCG	50.0	50.0	58.0	58.0	1513 bp	0.5	59.8
rpl20-rps12_p3	GAGTTCATTCTCCGGGGAAC	TGTTGATCTTGTAGCGGTCG	55.0	50.0	58.0	58.0	1523 bp	0.5	59.7
rpl20-rps12_p4	GTTCATTCTCCGGGGAACTC	TGTTGATCTTGTAGCGGTCG	55.0	50.0	58.0	58.0	1521 bp	0.5	59.7
rpl20-rps12_p5	TTCATTCTCCGGGGAACTCT	TGTTGATCTTGTAGCGGTCG	50.0	50.0	58.0	58.0	1520 bp	0.5	59.5