Hotspot

atpB-rbcL

LSC · 58868-59632 · 765 bp

Back to Meliosma

Pi 0.139

MarkerSeek score 83.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 100
pi: 0.139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATTCGTCCGAGAGTTGCTC	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1745 bp	0.5	59.9
atpB-rbcL_p2	AATTCGTCCGAGAGTTGCTC	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1319 bp	0.5	59.9
atpB-rbcL_p3	AATTCGTCCGAGAGTTGCTC	GGGGACGACCATACTTGTTC	50.0	55.0	58.0	58.0	1600 bp	0.5	59.8
atpB-rbcL_p4	TTTTCCTCCACGGCGATAAG	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1916 bp	0.5	59.8
atpB-rbcL_p5	TTTTCCTCCACGGCGATAAG	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1490 bp	0.5	59.8