Hotspot

ndhC-trnV-UAC

LSC · 55114-55862 · 749 bp

Back to Meliosma

Pi 0.36

MarkerSeek score 83.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 250
pi: 0.36
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTGTGCCGGCTGTTAAAATG	TAGGTTGATCTAGGCCGGAG	50.0	55.0	58.0	58.0	1706 bp	0.5	59.8
ndhC-trnV-UAC_p2	GAAACTCATCGCCCATGGAT	TAGGTTGATCTAGGCCGGAG	50.0	55.0	58.0	58.0	1301 bp	0.5	59.8
ndhC-trnV-UAC_p3	CTGTGCCGGCTGTTAAAATG	TTAGGCGCCAAATAGAACCC	50.0	50.0	58.0	58.0	1889 bp	0.5	59.5
ndhC-trnV-UAC_p4	GAAACTCATCGCCCATGGAT	TTAGGCGCCAAATAGAACCC	50.0	50.0	58.0	58.0	1484 bp	0.5	59.4
ndhC-trnV-UAC_p5	TGGTTCAGGCATTTGCTCAT	TAGGTTGATCTAGGCCGGAG	45.0	55.0	58.1	58.0	1762 bp	0.5	59.4