Hotspot

psaA-ycf3

LSC · 45720-46379 · 660 bp

Back to Meliosma

Pi 0.17

MarkerSeek score 83.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 104
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCAATTTCGGAGCTGCTTTG	GAATTGACTGCTGAGCCGTA	50.0	50.0	58.0	58.0	1448 bp	0.5	59.8
psaA-ycf3_p2	CCAATTTCGGAGCTGCTTTG	CTGAGCCGTATGAGGTAGGA	50.0	55.0	58.0	58.0	1438 bp	0.5	59.8
psaA-ycf3_p3	GCTGGGTCCAATGTGAGTAG	GAATTGACTGCTGAGCCGTA	55.0	50.0	58.0	58.0	1225 bp	0.5	59.7
psaA-ycf3_p4	CCAATTTCGGAGCTGCTTTG	GCGCTATTTTTGGCCGATAC	50.0	50.0	58.0	58.0	1876 bp	0.5	59.6
psaA-ycf3_p5	GCTGGGTCCAATGTGAGTAG	CTGAGCCGTATGAGGTAGGA	55.0	55.0	58.0	58.0	1215 bp	0.5	59.6