Hotspot

petN-psbM

LSC · 30811-32022 · 1212 bp

Back to Meliosma

Pi 0.257

MarkerSeek score 77.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 219
pi: 0.257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACGAATGTGTCTCCCGATTG	CGTCATCAGATGGGTGTGTT	50.0	50.0	58.0	57.9	2189 bp	0.5	59.1
petN-psbM_p2	CACGAATGTGTCTCCCGATT	CGTCATCAGATGGGTGTGTT	50.0	50.0	58.0	57.9	2190 bp	0.5	59.1
petN-psbM_p3	CGAATGTGTCTCCCGATTGT	CGTCATCAGATGGGTGTGTT	50.0	50.0	58.0	57.9	2188 bp	0.5	59.1
petN-psbM_p4	CTAACCCCCAGTCACGAATG	CGTCATCAGATGGGTGTGTT	55.0	50.0	58.0	57.9	2202 bp	0.5	59.0
petN-psbM_p5	AGTAAATCTCGCTTGGGCTG	CGTCATCAGATGGGTGTGTT	50.0	50.0	58.0	57.9	1757 bp	0.5	59.0