Hotspot

atpH-atpI

LSC · 15144-16321 · 1178 bp

Back to Meliosma

Pi 0.206

MarkerSeek score 82.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1178
variable_sites: 215
pi: 0.206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TATGGTCCTTGGGTTCCCTT	45.0	50.0	58.0	57.9	1684 bp	1.0	91.1
atpH-atpI_p2	GCCACGACCAGTCCATAAAT	TATGGTCCTTGGGTTCCCTT	50.0	50.0	58.0	57.9	1864 bp	0.5	59.1
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	ACAAACCATTCCTACCGACG	45.0	50.0	58.0	57.9	1772 bp	0.5	59.0
atpH-atpI_p4	GCCACGACCAGTCCATAAAT	ACAAACCATTCCTACCGACG	50.0	50.0	58.0	57.9	1952 bp	0.5	59.0
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CTGGTCAGGGGCTCTTTTAC	45.0	55.0	58.0	57.9	1628 bp	0.5	58.8