Hotspot

trnK-UUU-rps16

LSC · 4277-5160 · 884 bp

Back to Meliosma

Pi 0.334
MarkerSeek score 81.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
884
variable_sites
262
pi
0.334
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1311-1347 bp 1.0 91.3
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1310-1346 bp 1.0 91.3
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG ACCGAGAAAGGATCCTGGAA 55.0 50.0 58.0 58.0 1646 bp 0.5 59.5
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG ACCGAGAAAGGATCCTGGAA 55.0 50.0 58.0 58.0 1645 bp 0.5 59.5
trnK-UUU-rps16_p5 TCCCTTTCAGGATCAGTCGT ACCGAGAAAGGATCCTGGAA 50.0 50.0 58.1 58.0 1647 bp 0.5 59.1