Hotspot

ndhF

SSC · 104468-106684 · 2217 bp

Back to Melinis

Pi 0.00767

MarkerSeek score 47.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 17
pi: 0.00767
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGAGTGGGTTTGGTGAATCG	45.0	50.0	58.2	57.8	2352 bp	1.0	77.7
ndhF_p2	TGCATTTTGATGAGCCTCGT	TGGGTTTGGTGAATCGGAAA	45.0	45.0	58.2	57.6	2348 bp	1.0	76.3
ndhF_p3	TGCATTTTGATGAGCCTCGT	GTGGGTTTGGTGAATCGGAA	45.0	50.0	58.2	58.4	2349 bp	1.0	76.1
ndhF_p4	GTTTGCTGGCTTATTTGGCA	AGAGTGGGTTTGGTGAATCG	45.0	50.0	57.5	57.8	2511-2513 bp	1.0	75.5
ndhF_p5	ACGGAGACTTGCTTCACATT	AGAGTGGGTTTGGTGAATCG	45.0	50.0	57.4	57.8	2597-2599 bp	1.0	74.7