Hotspot

ndhG-ndhI

SSC · 112926-113108 · 183 bp

Back to Melinis

Pi 0.0166

MarkerSeek score 61.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 183
variable_sites: 3
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAGCGAAAAGGCAGAATA	GGTTACCGATCTCCATAATGGG	50.0	50.0	58.2	58.1	413-415 bp	1.0	62.5
ndhG-ndhI_p2	CCCAGCGAAAAGGCAGAATA	CGCCTCAAAGTAAACTAGACGA	50.0	45.5	58.2	58.2	362-364 bp	1.0	61.7
ndhG-ndhI_p3	CCCAGCGAAAAGGCAGAATA	TCGCCTCAAAGTAAACTAGACG	50.0	45.5	58.2	58.2	363-365 bp	1.0	61.7
ndhG-ndhI_p4	CCCAGCGAAAAGGCAGAATA	CGCCTCAAAGTAAACTAGACG	50.0	47.6	58.2	56.8	362-364 bp	1.0	61.2
ndhG-ndhI_p5	CCCAGCGAAAAGGCAGAATA	GTTACCGATCTCCATAATGGGA	50.0	45.5	58.2	57.1	412-414 bp	1.0	55.9