Hotspot

rps15-ndhF

IRb · 104341-104467 · 127 bp

Back to Melinis

Pi 0.0236

MarkerSeek score 66.10

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 127
variable_sites: 3
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	GTTTGCTGGCTTATTTGGCA	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.5	58.9	291-293 bp	1.0	69.7
rps15-ndhF_p2	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATAT	45.0	57.9	57.5	57.9	285-287 bp	1.0	68.5
rps15-ndhF_p3	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATATC	45.0	60.0	57.5	59.1	285-287 bp	1.0	68.4
rps15-ndhF_p4	CGTTTGCTGGCTTATTTGGC	AAATATGTAGGGGGAGGGCG	50.0	55.0	58.7	58.9	292-294 bp	1.0	68.1
rps15-ndhF_p5	ACGTTTGCTGGCTTATTTGG	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.3	58.9	293-295 bp	1.0	67.5