Hotspot

psaJ-rpl33

LSC · 66285-66626 · 342 bp

Back to Melinis

Pi 0.0117

MarkerSeek score 52.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 4
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	CCCCGTCGATTCCTTATTGG	45.0	55.0	57.9	58.1	510 bp	1.0	78.9
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	GAAATCCCCGTCGATTCCTT	45.0	50.0	57.9	57.7	515 bp	1.0	77.2
psaJ-rpl33_p3	ATGGTTTGGGGCTTTAGCAG	CCCCGTCGATTCCTTATTGG	50.0	55.0	58.4	58.1	514 bp	1.0	76.1
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CCCGTCGATTCCTTATTGGT	45.0	50.0	57.9	57.4	509 bp	1.0	74.9
psaJ-rpl33_p5	ATGGTTTGGGGCTTTAGCAG	GAAATCCCCGTCGATTCCTT	50.0	50.0	58.4	57.7	519 bp	1.0	74.5