Hotspot

psbE-petL

LSC · 63681-64965 · 1285 bp

Back to Melinis

Pi 0.00545

MarkerSeek score 61.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 7
pi: 0.00545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1443-1445 bp	1.0	76.4
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1441-1443 bp	1.0	76.4
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1432-1434 bp	1.0	76.2
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1430-1432 bp	1.0	76.1
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1434-1436 bp	1.0	74.7