Hotspot

petA-psbJ

LSC · 62011-62912 · 902 bp

Back to Melinis

Pi 0.00887

MarkerSeek score 63.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 8
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TGGTTCGTATTCTGGATTGGG	50.0	47.6	57.9	57.7	1038 bp	1.0	70.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	1037 bp	1.0	70.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	TGGTTCGTATTCTGGATTGGG	50.0	47.6	58.7	57.7	1045 bp	1.0	65.0
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	1044 bp	1.0	64.9
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	TGGTTCGTATTCTGGATTGGG	45.0	47.6	57.2	57.7	1037 bp	1.0	64.2