Hotspot

ycf3-trnS-GGA

LSC · 45965-46553 · 589 bp

Back to Melinis

Pi 0.00343

MarkerSeek score 61.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 2
pi: 0.00343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGGCTACAATCGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.0	58.2	729-735 bp	1.0	72.0
ycf3-trnS-GGA_p2	TGGCTACAATCGAAGAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.0	57.8	679-685 bp	1.0	71.7
ycf3-trnS-GGA_p3	TTGGCTACAATCGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.8	58.2	730-736 bp	1.0	69.7
ycf3-trnS-GGA_p4	GGCTACAATCGAAGAGGTCT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	56.7	58.2	728-734 bp	1.0	69.6
ycf3-trnS-GGA_p5	TTGGCTACAATCGAAGAGGTC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.8	57.8	680-686 bp	1.0	69.4