Hotspot

trnR-UCU-trnfM-CAU

LSC · 37974-38145 · 172 bp

Back to Melinis

Pi 0.0233

MarkerSeek score 60.50

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 4
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	GGCGTATCCAATCCCCTTTT	47.6	50.0	56.9	57.9	304-306 bp	1.0	64.1
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	GGCGTATCCAATCCCCTTTT	45.5	50.0	57.9	57.9	310-312 bp	1.0	63.4
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	GGATATGGTAGAGGGGCGTA	47.6	55.0	56.9	57.8	318-320 bp	1.0	62.8
trnR-UCU-trnfM-CAU_p4	AGAAGACCTCTGTCCTATCCA	TATGGTAGAGGGGCGTATCC	47.6	55.0	56.9	57.8	315-317 bp	1.0	62.8
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCA	AATTTGGTAGCTCACGAGGC	47.6	50.0	56.9	58.3	255-257 bp	1.0	62.7