Hotspot

trnC-GCA-rpoB

LSC · 20066-21227 · 1162 bp

Back to Melinis

Pi 0.0104

MarkerSeek score 57.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 12
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	TTATTGATTAGGCGGCACCC	CCGGGTATTGTGGACATTCC	50.0	55.0	58.0	58.3	1282-1283 bp	1.0	78.6
trnC-GCA-rpoB_p2	TTATTGATTAGGCGGCACCC	CGGGTATTGTGGACATTCCC	50.0	55.0	58.0	58.3	1281-1282 bp	1.0	78.6
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1245-1246 bp	1.0	78.2
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1244-1245 bp	1.0	78.2
trnC-GCA-rpoB_p5	TTATTGATTAGGCGGCACCC	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.0	57.6	1346-1347 bp	1.0	78.1