Hotspot

psbK-psbI

LSC · 7450-7847 · 398 bp

Back to Melinis

Pi 0.0504

MarkerSeek score 77.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 20
pi: 0.0504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	536-539 bp	1.0	81.3
psbK-psbI_p2	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	591-594 bp	1.0	81.2
psbK-psbI_p3	AGCCGCTGTAAGTTTTCGAT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	534-537 bp	1.0	80.9
psbK-psbI_p4	AAGCCGCTGTAAGTTTTCGA	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.1	57.8	535-538 bp	1.0	80.7
psbK-psbI_p5	ACCCGAAGCTTATGCCATTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	621-624 bp	1.0	80.1