Hotspot

ycf2

Genome · 87195-92813 · 5619 bp

Back to Melilotus

Pi 0.478

MarkerSeek score 72.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 5619
variable_sites: 2129
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGGATTTGACCCCATAAGCG	AGGATTTTCCGCAAAGTGGT	50.0	45.0	57.9	58.0	1029 bp	0.5	59.6
ycf2_p2	TGGATTTGACCCCATAAGCG	TTTCCGCAAAGTGGTGATGA	50.0	45.0	57.9	58.0	1024 bp	0.5	59.4
ycf2_p3	ATATCTTCGCACACGGACAC	AGGATTTTCCGCAAAGTGGT	50.0	45.0	58.1	58.0	658 bp	0.5	59.3
ycf2_p4	AATTCGCTCGGATCTATGCC	AGGATTTTCCGCAAAGTGGT	50.0	45.0	57.9	58.0	563 bp	0.5	59.3
ycf2_p5	TCGCTCGGATCTATGCCTAA	AGGATTTTCCGCAAAGTGGT	50.0	45.0	58.1	58.0	560 bp	0.5	59.2