Hotspot

trnI-CAU

Genome · 92930-93003 · 74 bp

Back to Melilotus

Pi 0.548

MarkerSeek score 73.80

Primer available yes

Label trnI-CAU

Feature Metadata

label_name: trnI-CAU
feature_type: tRNA
region: Genome
length_bp: 74
variable_sites: 40
pi: 0.548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU_p1	AAACCCTGTAAATGGCGTGA	TTTCGAGGCGATCGGAAAAT	45.0	45.0	57.7	58.0	615 bp	0.5	57.6
trnI-CAU_p2	AACCCTGTAAATGGCGTGAA	TTTCGAGGCGATCGGAAAAT	45.0	45.0	57.7	58.0	614 bp	0.5	57.6
trnI-CAU_p3	ACCCTGTAAATGGCGTGAAA	TTTCGAGGCGATCGGAAAAT	45.0	45.0	57.7	58.0	613 bp	0.5	57.6
trnI-CAU_p4	TTGACGAGACAGGAGTGGAA	TTTCGAGGCGATCGGAAAAT	50.0	45.0	58.3	58.0	853 bp	0.5	57.4
trnI-CAU_p5	TTTGACGAGACAGGAGTGGA	TTTCGAGGCGATCGGAAAAT	50.0	45.0	58.3	58.0	854 bp	0.5	57.4