Hotspot

clpP

Genome · 67980-71087 · 3108 bp

Back to Melilotus

Pi 0.289

MarkerSeek score 78.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 3108
variable_sites: 793
pi: 0.289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGCATATGTTACAAGGGCCG	AGGATTAGCCGCTTCAATGG	50.0	50.0	58.0	58.0	2004 bp	0.5	59.5
clpP_p2	CCTGCTGGAGATGGACAATC	AGGATTAGCCGCTTCAATGG	55.0	50.0	58.0	58.0	1807 bp	0.5	59.5
clpP_p3	ATCAACAATTCCGTGGGCTT	AGGATTAGCCGCTTCAATGG	45.0	50.0	58.1	58.0	2797 bp	0.5	59.2
clpP_p4	CTTGTCCTTGTGCTTGTCCT	AGCACAAGGACAAGCACAAT	50.0	45.0	58.0	58.3	328 bp	0.5	57.4
clpP_p5	AGCATATGTTACAAGGGCCG	AGCACAAGGACAAGCACAAT	50.0	45.0	58.0	58.3	805 bp	0.5	57.3