Hotspot

ycf1-rps15

Genome · 113400-113911 · 512 bp

Back to Melilotus

Pi 0.0804

MarkerSeek score 80.10

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 512
variable_sites: 39
pi: 0.0804
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GAACTGTGATCCTTCCGTCC	ACAGTAGTCGTTGCCGTTTT	55.0	45.0	58.0	58.1	1387-1424 bp	1.0	83.6
ycf1-rps15_p2	CTGTGATCCTTCCGTCCAAG	ACAGTAGTCGTTGCCGTTTT	55.0	45.0	58.0	58.1	1384-1421 bp	1.0	83.6
ycf1-rps15_p3	GAACTGTGATCCTTCCGTCC	AACACCATGCATTGATGGGT	55.0	45.0	58.0	58.1	1639-1676 bp	1.0	83.0
ycf1-rps15_p4	GAACTGTGATCCTTCCGTCC	ACCATGCATTGATGGGTGTT	55.0	45.0	58.0	58.1	1636-1673 bp	1.0	83.0
ycf1-rps15_p5	CTGTGATCCTTCCGTCCAAG	ACCATGCATTGATGGGTGTT	55.0	45.0	58.0	58.1	1633-1670 bp	1.0	82.9