Hotspot

trnN-GUU-ycf1

Genome · 106965-107509 · 545 bp

Back to Melilotus

Pi 0.0704

MarkerSeek score 75.20

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: Genome
length_bp: 545
variable_sites: 33
pi: 0.0704
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GAACGCTTTGCCTTTCCTTC	CTGTCCCGTAATAAACCCGG	50.0	55.0	57.9	58.1	869-888 bp	1.0	86.8
trnN-GUU-ycf1_p2	ATCCCCGGACTGTAGAACAA	CTGTCCCGTAATAAACCCGG	50.0	55.0	58.1	58.1	1023-1042 bp	1.0	86.4
trnN-GUU-ycf1_p3	GAACGCTTTGCCTTTCCTTC	CCCGTAATAAACCCGGTTGT	50.0	50.0	57.9	57.9	865-884 bp	1.0	86.3
trnN-GUU-ycf1_p4	CCCCGGACTGTAGAACAAAG	CTGTCCCGTAATAAACCCGG	55.0	55.0	57.9	58.1	1021-1040 bp	1.0	86.1
trnN-GUU-ycf1_p5	CCCGGACTGTAGAACAAAGG	CTGTCCCGTAATAAACCCGG	55.0	55.0	57.9	58.1	1020-1039 bp	1.0	86.1