Hotspot

trnI-CAU-ndhB

Genome · 93004-94095 · 1092 bp

Back to Melilotus

Pi 0.343

MarkerSeek score 83.00

Primer available yes

Label trnI-CAU-ndhB

Feature Metadata

label_name: trnI-CAU-ndhB
feature_type: igs
region: Genome
length_bp: 1092
variable_sites: 348
pi: 0.343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ndhB_p1	ATTGGCGAAGTCGTAGGTTC	AACGGTGGATATGCAAGCAT	50.0	45.0	58.0	57.9	1630 bp	0.5	59.5
trnI-CAU-ndhB_p2	GGCGAAGTCGTAGGTTCAAT	AACGGTGGATATGCAAGCAT	50.0	45.0	58.0	57.9	1627 bp	0.5	59.5
trnI-CAU-ndhB_p3	GAATGGTTAAAGCGCCCAAC	AACGGTGGATATGCAAGCAT	50.0	45.0	58.0	57.9	1655 bp	0.5	59.5
trnI-CAU-ndhB_p4	ATTGGCGAAGTCGTAGGTTC	AGAAATAACCCCTCACGTGC	50.0	50.0	58.0	57.9	1283 bp	0.5	59.1
trnI-CAU-ndhB_p5	GGCGAAGTCGTAGGTTCAAT	AGAAATAACCCCTCACGTGC	50.0	50.0	58.0	57.9	1280 bp	0.5	59.1