Hotspot

rpl23-trnL-CAA

Genome · 86287-86604 · 318 bp

Back to Melilotus

Pi 0.19
MarkerSeek score 76.80
Primer available yes
Label rpl23-trnL-CAA

Feature Metadata

label_name
rpl23-trnL-CAA
feature_type
igs
region
Genome
length_bp
318
variable_sites
58
pi
0.19
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnL-CAA_p1 AAACCGCACTCAAAGGTAGG GTCTCGCGTGTCTACCATTT 50.0 50.0 58.1 58.0 1052 bp 0.5 59.1
rpl23-trnL-CAA_p2 AAACCGCACTCAAAGGTAGG CTCGCGTGTCTACCATTTCA 50.0 50.0 58.1 58.0 1050 bp 0.5 59.1
rpl23-trnL-CAA_p3 AAACCGCACTCAAAGGTAGG GTGATCTGCTGGCGAACTTA 50.0 50.0 58.1 58.0 1150 bp 0.5 59.1
rpl23-trnL-CAA_p4 CCCTGCGGTAATGATTCCTC GTCTCGCGTGTCTACCATTT 55.0 50.0 58.1 58.0 802 bp 0.5 59.0
rpl23-trnL-CAA_p5 CCCTGCGGTAATGATTCCTC CTCGCGTGTCTACCATTTCA 55.0 50.0 58.1 58.0 800 bp 0.5 59.0