Hotspot

petB-petD

Genome · 75466-75660 · 195 bp

Back to Melilotus

Pi 0.0703

MarkerSeek score 75.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: Genome
length_bp: 195
variable_sites: 13
pi: 0.0703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TAAGCAAGGCATTTCCGGTC	GTTCAAGTCACACACTCCCA	50.0	50.0	58.5	57.7	243-244 bp	1.0	80.6
petB-petD_p2	CAAGGCATTTCCGGTCCTTT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.7	57.7	239-240 bp	1.0	79.1
petB-petD_p3	GTAAGCAAGGCATTTCCGGT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.8	57.7	244-245 bp	1.0	78.3
petB-petD_p4	TAAGCAAGGCATTTCCGGTC	AGTTCAAGTCACACACTCCC	50.0	50.0	58.5	57.4	244-245 bp	1.0	78.2
petB-petD_p5	AAGGCATTTCCGGTCCTTTA	GTTCAAGTCACACACTCCCA	45.0	50.0	57.1	57.7	238-239 bp	1.0	78.0