Hotspot

clpP-psbB

Genome · 71088-71464 · 377 bp

Back to Melilotus

Pi 0.0567
MarkerSeek score 73.60
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
Genome
length_bp
377
variable_sites
20
pi
0.0567
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CCATTGAAGCGGCTAATCCT AACAGGGTCAGAGGGATCAA 50.0 50.0 58.0 58.0 852-867 bp 1.0 86.1
clpP-psbB_p2 CCATTGAAGCGGCTAATCCT ACAGGGTCAGAGGGATCAAA 50.0 50.0 58.0 58.0 851-866 bp 1.0 86.1
clpP-psbB_p3 CCATTGAAGCGGCTAATCCT AAAAGCACAAGCCCGAAAAC 50.0 45.0 58.0 58.1 1024-1039 bp 1.0 84.9
clpP-psbB_p4 CCATTGAAGCGGCTAATCCT AATGCGCCAAAACCAAAACA 50.0 40.0 58.0 58.0 1166-1181 bp 1.0 84.5
clpP-psbB_p5 CCATTGAAGCGGCTAATCCT GCTTGTACCCTTCCGGTTAG 50.0 55.0 58.0 58.0 1238-1253 bp 1.0 84.3