Hotspot

ycf1

SSC · 126827-132295 · 5469 bp

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Pi 0.0103

MarkerSeek score 56.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 56
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCATAACGTTTTGATTCTTCGA	AGGATACCACGAAAAAGGCT	39.1	45.0	58.0	56.8	192 bp	1.0	48.8
ycf1_p2	GCCATAACGTTTTGATTCTTCGA	AAGGATACCACGAAAAAGGCT	39.1	42.9	58.0	57.6	193 bp	1.0	46.7
ycf1_p3	GCCATAACGTTTTGATTCTTCG	AGGATACCACGAAAAAGGCT	40.9	45.0	56.7	56.8	192 bp	1.0	46.6
ycf1_p4	GCCATAACGTTTTGATTCTTCG	AAGGATACCACGAAAAAGGCT	40.9	42.9	56.7	57.6	193 bp	1.0	44.5
ycf1_p5	GCCATAACGTTTTGATTCTTCGA	ACCACGAAAAAGGCTATGTTG	39.1	42.9	58.0	57.1	187 bp	1.0	43.0