Hotspot

rps16

LSC · 4900-6065 · 1166 bp

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Pi 0.0155

MarkerSeek score 53.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1166
variable_sites: 18
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCCGTACTTAAAAGCCGAG	CAATTGAGACGGGGGTGATT	50.0	50.0	58.0	57.9	2220-2227 bp	1.0	82.0
rps16_p2	CCTTTCAGGATCAGTCGTGG	CAATTGAGACGGGGGTGATT	55.0	50.0	58.0	57.9	2304-2311 bp	1.0	81.8
rps16_p3	AGCCGTACTTAAAAGCCGAG	TGAGACGGGGGTGATTCATA	50.0	50.0	58.0	57.8	2216-2223 bp	1.0	81.7
rps16_p4	CCTTTCAGGATCAGTCGTGG	TGAGACGGGGGTGATTCATA	55.0	50.0	58.0	57.8	2300-2307 bp	1.0	81.5
rps16_p5	CTCTCCCGAAGATTTGGACG	CAATTGAGACGGGGGTGATT	55.0	50.0	58.1	57.9	2275-2282 bp	1.0	81.4