Hotspot

rpl16

LSC · 83244-84624 · 1381 bp

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Pi 0.0101

MarkerSeek score 50.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1381
variable_sites: 14
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	TGACGGAAAAGAAATTGCGC	50.0	45.0	58.0	58.0	2126-2165 bp	1.0	80.5
rpl16_p2	ACCAAAAACTCGAGTTCCCC	TGACGGAAAAGAAATTGCGC	50.0	45.0	58.0	58.0	2123-2162 bp	1.0	80.5
rpl16_p3	CGCACCAAAAACTCGAGTTC	CACCCCAATATTCTTGCCGA	50.0	50.0	58.0	57.9	2263-2302 bp	1.0	80.4
rpl16_p4	ACCAAAAACTCGAGTTCCCC	CACCCCAATATTCTTGCCGA	50.0	50.0	58.0	57.9	2260-2299 bp	1.0	80.4
rpl16_p5	CGCACCAAAAACTCGAGTTC	TACAAATTGCAGGGCGTCTT	50.0	45.0	58.0	58.1	2145-2184 bp	1.0	80.1