Hotspot

ndhG

SSC · 121164-121700 · 537 bp

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Pi 0.0223

MarkerSeek score 57.90

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 537
variable_sites: 12
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGGTATTGCTGTTTCTAGTTCT	AAATGCCTGGTCGGTTCAAT	36.4	45.0	55.1	58.1	635 bp	1.0	41.9
ndhG_p2	TGGTATTGCTGTTTCTAGTTCT	AAAAATGCCTGGTCGGTTCA	36.4	45.0	55.1	58.3	637 bp	1.0	41.6
ndhG_p3	TGGTATTGCTGTTTCTAGTTCT	AAAATGCCTGGTCGGTTCAA	36.4	45.0	55.1	58.3	636 bp	1.0	41.6
ndhG_p4	TGGTATTGCTGTTTCTAGTTCT	TGCCTGGTCGGTTCAATAAG	36.4	50.0	55.1	57.9	632 bp	1.0	41.5
ndhG_p5	TGGTATTGCTGTTTCTAGTTCT	GCCTGGTCGGTTCAATAAGT	36.4	50.0	55.1	57.9	631 bp	1.0	41.5