Hotspot

rpl32-trnL-UAG

SSC · 115989-117002 · 1014 bp

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Pi 0.0169
MarkerSeek score 69.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1014
variable_sites
17
pi
0.0169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGAGGGTATTGGTCAACA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1147-1153 bp 1.0 81.1
rpl32-trnL-UAG_p2 AGGGAGGGTATTGGTCAACA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1148-1154 bp 1.0 81.1
rpl32-trnL-UAG_p3 AGGGAGGGTATTGGTCAACA CTCGCACTGCTTCCTAAGAG 50.0 55.0 57.9 58.1 1168-1174 bp 1.0 80.5
rpl32-trnL-UAG_p4 AGGGAGGGTATTGGTCAACA CTCTCGCACTGCTTCCTAAG 50.0 55.0 57.9 58.1 1170-1176 bp 1.0 80.5
rpl32-trnL-UAG_p5 AGGGAGGGTATTGGTCAACA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1157-1163 bp 1.0 80.2