Hotspot

trnN-GUU-rpl32

SSC · 111218-115814 · 4597 bp

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Pi 0.00765
MarkerSeek score 58.00
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
4597
variable_sites
35
pi
0.00765
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGCATGAATTCCACTTTCGC TGGAATTACGAATGGGGTCG 45.0 50.0 57.1 57.7 282-284 bp 1.0 75.7
trnN-GUU-rpl32_p2 GCATGAATTCCACTTTCGCT TGGAATTACGAATGGGGTCG 45.0 50.0 57.1 57.7 281-283 bp 1.0 75.7
trnN-GUU-rpl32_p3 AGCATGAATTCCACTTTCGC GAATTACGAATGGGGTCGGT 45.0 50.0 57.1 57.7 280-282 bp 1.0 75.7
trnN-GUU-rpl32_p4 GCATGAATTCCACTTTCGCT GAATTACGAATGGGGTCGGT 45.0 50.0 57.1 57.7 279-281 bp 1.0 75.7
trnN-GUU-rpl32_p5 GCATGAATTCCACTTTCGCT GGAATTACGAATGGGGTCGG 45.0 55.0 57.1 58.4 280-282 bp 1.0 75.0