Hotspot

rpl16-rps3

LSC · 84625-84785 · 161 bp

Back to Melicope

Pi 0.0437

MarkerSeek score 65.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 7
pi: 0.0437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GAGGGCAGAGTTCCTCTACA	45.0	55.0	57.6	58.1	306-307 bp	1.0	77.7
rpl16-rps3_p2	AAACCAACGAGTCACACACT	AGGGCAGAGTTCCTCTACAA	45.0	50.0	57.6	57.7	305-306 bp	1.0	76.3
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	GAGGGCAGAGTTCCTCTACA	45.0	55.0	57.1	58.1	301-302 bp	1.0	73.9
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	AGGGCAGAGTTCCTCTACAA	45.0	50.0	57.1	57.7	300-301 bp	1.0	72.4
rpl16-rps3_p5	ACCAACGAGTCACACACTAAG	GAGGGCAGAGTTCCTCTACA	47.6	55.0	57.9	58.1	304-305 bp	1.0	72.2