Hotspot

psbE-petL

LSC · 66472-67817 · 1346 bp

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Pi 0.0126

MarkerSeek score 54.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 17
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCCTATCTTGCTCAGACCA	50.0	47.6	57.8	57.9	1462-1480 bp	1.0	70.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGTCCTATCTTGCTCAGACCA	55.0	47.6	58.2	57.9	1457-1475 bp	1.0	70.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.8	56.7	1416-1434 bp	1.0	69.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.8	56.7	1415-1433 bp	1.0	69.7
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGGAGAAAACCAA	55.0	45.0	58.2	56.7	1410-1428 bp	1.0	69.4