Hotspot

psbC-trnS-UGA

LSC · 36868-37116 · 249 bp

Back to Melicope

Pi 0.0321

MarkerSeek score 71.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 8
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCTGCAGCAGGTTTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.1	58.1	398 bp	1.0	80.6
psbC-trnS-UGA_p2	GCTGCAGCAGGTTTTGAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.1	58.2	344 bp	1.0	79.6
psbC-trnS-UGA_p3	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	356 bp	1.0	79.2
psbC-trnS-UGA_p4	CCTGTTCTTTCGATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.3	58.2	302 bp	1.0	78.3
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	433 bp	1.0	77.6