Hotspot

trnT-GGU-psbD

LSC · 33195-34439 · 1245 bp

Back to Melicope

Pi 0.0113

MarkerSeek score 61.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 14
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CCGTAATTGAACCAAACGGC	AAAAACGAATCGGTCCCTCC	50.0	50.0	58.0	58.2	1425-1441 bp	1.0	79.2
trnT-GGU-psbD_p2	CGTAATTGAACCAAACGGCC	AAAAACGAATCGGTCCCTCC	50.0	50.0	58.0	58.2	1424-1440 bp	1.0	79.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	AAAAACGAATCGGTCCCTCC	55.0	50.0	58.1	58.2	1393-1409 bp	1.0	78.9
trnT-GGU-psbD_p4	TGAACCAAACGGCCCTTTTA	AAAAACGAATCGGTCCCTCC	45.0	50.0	57.9	58.2	1418-1434 bp	1.0	78.8
trnT-GGU-psbD_p5	ACCAAACGGCCCTTTTAACT	AAAAACGAATCGGTCCCTCC	45.0	50.0	57.9	58.2	1415-1431 bp	1.0	78.8