Hotspot

atpH-atpI

LSC · 14041-15060 · 1020 bp

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Pi 0.00787

MarkerSeek score 63.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 8
pi: 0.00787
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1104-1107 bp	1.0	79.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGACATC	45.0	55.0	58.0	57.8	1074-1077 bp	1.0	79.2
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1160-1163 bp	1.0	78.9
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGACATC	50.0	55.0	57.9	57.8	1130-1133 bp	1.0	78.5
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1105-1108 bp	1.0	78.3