Hotspot

atpA-atpF

LSC · 11921-11980 · 60 bp

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Pi 0.0333

MarkerSeek score 71.70

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 2
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	GCGCTCGGAACTCTGAATAG	55.0	55.0	58.1	58.5	275 bp	1.0	75.7
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	GCGCTCGGAACTCTGAATAG	55.0	55.0	58.1	58.5	273 bp	1.0	75.7
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	CAATATTGGCATGCTTGGGG	55.0	50.0	58.1	57.5	216 bp	1.0	75.7
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	CAATATTGGCATGCTTGGGG	55.0	50.0	58.1	57.5	214 bp	1.0	75.7
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	CCAATATTGGCATGCTTGGG	55.0	50.0	58.1	57.5	215 bp	1.0	75.7