Hotspot

rps16-trnQ-UUG

LSC · 6066-6888 · 823 bp

Back to Melicope

Pi 0.014

MarkerSeek score 71.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 11
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	882-917 bp	1.0	79.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	891-926 bp	1.0	77.9
rps16-trnQ-UUG_p3	CGGATCGTGTCCTTCAAGTC	GGGTTTTGGTCCCGGTATTC	55.0	55.0	58.4	58.5	897-932 bp	1.0	77.2
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GGGTTTTGGTCCCGGTATTC	55.0	55.0	58.4	58.5	896-931 bp	1.0	77.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AAATAGAAATGGGGCGTGGC	45.0	50.0	58.1	58.9	918-953 bp	1.0	76.1