Hotspot

ndhF

SSC · 101371-103590 · 2220 bp

Back to Melica

Pi 0.0041
MarkerSeek score 47.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
123
pi
0.0041
unique_haplotype_count
53
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACCCATCAATGCATGGTGTT AGAGAGCCGCTTCATCTTTG 45.0 50.0 58.1 58.0 2738-2845 bp 0.916667 85.4
ndhF_p2 ATCGCGGAAAACCGATGTAA AGAGAGCCGCTTCATCTTTG 45.0 50.0 58.0 58.0 2694-2722 bp 0.1 45.1
ndhF_p3 ATCGCGGAAAACCGATGTAA CAAGAGAGCCGCTTCATCTT 45.0 50.0 58.0 58.0 2696-2724 bp 0.1 45.1
ndhF_p4 AATCGCGGAAAACCGATGTA AGAGAGCCGCTTCATCTTTG 45.0 50.0 58.0 58.0 2703-2723 bp 0.05 41.9
ndhF_p5 AATCGCGGAAAACCGATGTA CAAGAGAGCCGCTTCATCTT 45.0 50.0 58.0 58.0 2705-2725 bp 0.05 41.9