Hotspot

psaC-ndhE

SSC · 108447-108911 · 465 bp

Back to Melica

Pi 0.00839
MarkerSeek score 69.20
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
465
variable_sites
59
pi
0.00839
unique_haplotype_count
41
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 GCTTTACATCCATCCCAGGG AGCCGCTATTGGACTATCCA 55.0 50.0 58.0 58.3 650-668 bp 1.0 89.4
psaC-ndhE_p2 GGCTTTACATCCATCCCAGG AGCCGCTATTGGACTATCCA 55.0 50.0 58.0 58.3 651-669 bp 1.0 89.4
psaC-ndhE_p3 GTTGGACAAGCTCGTACACA AGCCGCTATTGGACTATCCA 50.0 50.0 58.1 58.3 611-629 bp 1.0 88.6
psaC-ndhE_p4 TGTTGGACAAGCTCGTACAC AGCCGCTATTGGACTATCCA 50.0 50.0 58.1 58.3 612-630 bp 1.0 88.6
psaC-ndhE_p5 CTGTTGGACAAGCTCGTACA AGCCGCTATTGGACTATCCA 50.0 50.0 57.9 58.3 613-631 bp 1.0 88.5