Hotspot

psbT-psbN

LSC · 70498-70545 · 48 bp

Back to Melica

Pi 0.0372
MarkerSeek score 63.60
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
48
variable_sites
6
pi
0.0372
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTCCGAGAACCACCTAAGGT TCGCCATCTCCATATCTGGT 50.0 50.0 58.0 58.0 203 bp 1.0 84.1
psbT-psbN_p2 TTCCGAGAACCACCTAAGGT CCATTCGAAGAACACGGAGA 50.0 50.0 58.0 57.6 114 bp 1.0 82.5
psbT-psbN_p3 TTCCGAGAACCACCTAAGGT TCCATTCGAAGAACACGGAG 50.0 50.0 58.0 57.6 115 bp 1.0 82.5
psbT-psbN_p4 TTCCGAGAACCACCTAAGGT GTCGCCATCTCCATATCTGG 50.0 55.0 58.0 57.6 204 bp 1.0 81.1
psbT-psbN_p5 ACCACCTAAGGTTCCGACTA TCGCCATCTCCATATCTGGT 50.0 50.0 57.4 58.0 195 bp 0.966667 77.4