Hotspot

rbcL-psaI

LSC · 56441-57424 · 984 bp

Back to Melica

Pi 0.0105
MarkerSeek score 79.90
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
984
variable_sites
105
pi
0.0105
unique_haplotype_count
56
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 TCGAGTTCGAGCCAGTAGAT AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 972-1140 bp 1.0 90.9
rbcL-psaI_p2 TCGAGTTCGAGCCAGTAGAT CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 966-1134 bp 0.983333 90.6
rbcL-psaI_p3 AATGGAGTCCTGAACTTGCC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.8 57.9 1023-1191 bp 1.0 89.3
rbcL-psaI_p4 AATGGAGTCCTGAACTTGCC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 1017-1185 bp 0.983333 89.1
rbcL-psaI_p5 CGCAGCTTGTGAAGTATGGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.3 58.0 998-1166 bp 0.983333 88.7