Hotspot

ndhC-trnV

LSC · 50420-51272 · 853 bp

Back to Melica

Pi 0.00696
MarkerSeek score 70.30
Primer available yes
Label ndhC-trnV

Feature Metadata

label_name
ndhC-trnV
feature_type
igs
region
LSC
length_bp
853
variable_sites
71
pi
0.00696
unique_haplotype_count
46
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_p1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.0 57.9 898-1034 bp 1.0 89.2
ndhC-trnV_p2 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.0 58.3 899-1035 bp 1.0 87.3
ndhC-trnV_p3 TCTGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 926-1062 bp 1.0 83.4
ndhC-trnV_p4 CTTCACTAACCGGGGCTAAA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 919-1055 bp 1.0 83.1
ndhC-trnV_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 917-1053 bp 1.0 83.1