Hotspot

psaA-ycf3

LSC · 41853-42505 · 653 bp

Back to Melica

Pi 0.00859
MarkerSeek score 70.60
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
653
variable_sites
62
pi
0.00859
unique_haplotype_count
57
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GTCTGGCCCATTCCTCAAAA TATAGCGCTTACTCCGGGAA 50.0 50.0 58.1 58.0 795-814 bp 1.0 88.8
psaA-ycf3_p2 GTCTGGCCCATTCCTCAAAA GCGCTTACTCCGGGAAATTA 50.0 50.0 58.1 58.1 791-810 bp 1.0 88.5
psaA-ycf3_p3 CTGGCCCATTCCTCAAAAGA TATAGCGCTTACTCCGGGAA 50.0 50.0 57.8 58.0 793-812 bp 1.0 87.6
psaA-ycf3_p4 TCTGGCCCATTCCTCAAAAG TATAGCGCTTACTCCGGGAA 50.0 50.0 57.8 58.0 794-813 bp 1.0 87.6
psaA-ycf3_p5 CTGGCCCATTCCTCAAAAGA GCGCTTACTCCGGGAAATTA 50.0 50.0 57.8 58.1 789-808 bp 1.0 87.4