Hotspot

trnR-rps14

LSC · 36530-36907 · 378 bp

Back to Melica

Pi 0.0109
MarkerSeek score 66.60
Primer available yes
Label trnR-rps14

Feature Metadata

label_name
trnR-rps14
feature_type
igs
region
LSC
length_bp
378
variable_sites
38
pi
0.0109
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-rps14_p1 AGAAGACCTCTGTCCTATCCA TTACCGGGTGCAACAAGATC 47.6 50.0 56.9 58.2 436-444 bp 1.0 72.3
trnR-rps14_p2 AGAAGACCTCTGTCCTATCCA TCCGGACACATACTTCGAGA 47.6 50.0 56.9 57.9 475-483 bp 1.0 72.0
trnR-rps14_p3 AGGTTTAGAAGACCTCTGTCCT TTACCGGGTGCAACAAGATC 45.5 50.0 57.9 58.2 442-450 bp 1.0 71.5
trnR-rps14_p4 AGAAGACCTCTGTCCTATCCA TTGGGCTATCCGGACACATA 47.6 50.0 56.9 58.2 483-491 bp 1.0 71.3
trnR-rps14_p5 AGGTTTAGAAGACCTCTGTCCT TCCGGACACATACTTCGAGA 45.5 50.0 57.9 57.9 481-489 bp 1.0 71.2